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Literature summary extracted from

  • Simon, N.; Vergis, J.; Ebrahimi, A.; Ventura, C.; O'Brien, A.; Barbieri, J.
    Host cell cytotoxicity and cytoskeleton disruption by CerADPr, an ADP-ribosyltransferase of Bacillus cereus G9241 (2013), Biochemistry, 52, 2309-2318.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.30 expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.30 E431D inactive Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.30 29451
-
 4 * 29451, calculated from amino acid sequence Bacillus cereus
2.4.2.30 30000
-
 4 * 30000, SDS-PAGE Bacillus cereus
2.4.2.30 120000
-
 gel filtration Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Bacillus cereus
-
-
-
2.4.2.30 Bacillus cereus G9241
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.30 Ni2+ affinity resin column chromatography and Sephacryl S200 gel filtration Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-biotin
-
 Bacillus cereus nicotinamide + (ADP-D-ribosyl)n+1-biotin + H+
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-biotin
-
 Bacillus cereus G9241 nicotinamide + (ADP-D-ribosyl)n+1-biotin + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.30 homotetramer 4 * 30000, SDS-PAGE Bacillus cereus
2.4.2.30 homotetramer 4 * 29451, calculated from amino acid sequence Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.30 ADP-ribosyltransferase
-
 Bacillus cereus
2.4.2.30 ADPr
-
 Bacillus cereus

General Information

EC Number General Information Comment Organism
2.4.2.30 physiological function the enzyme disrupts host cytoskeletal signaling Bacillus cereus